Electrophysiology Laboratory

Responsabile: Ruffolo Gabriele
Staff: Alfano Veronica

*In collaboration with Prof. Eleonora Palma (Full Professor of Physiology, University of Rome “Sapienza”)

The electrophysiology laboratory has all the ‘equipment necessary to conduct voltage-clamp recordings in Xenopus oocytes.The setup consists of two micromanipulators (Leica Microsystems), a Warner Instruments RC-1Z recording chamber and a modular stereomicroscope (Leica MS5), and acquires via GeneClamp 500B amplifier associated with a Digidata 1322A (Axon Instruments), which allow digitization of the recorded signal. The aforementioned instrumentation also allows for perfusions of oocytes, thanks to the Warner VC-8 system, in order to test the effect of different modulators on neurotransmitter receptors.Finally, the laboratory has an antivibratility system (Kinetic system 9100) and a Faraday cage to minimize electrical noise while conducting experiments.

Interessi di Ricerca

The Neurophysiology laboratory is mainly concerned with the following topics:
  • Physiology of neurodevelopment
  • Synaptic transmission in normal and pathological muscle

Pubblicazioni

  • Gabriele Ruffolo, Alessandro Gaeta, Beatrice Cannata, Camilla Pinzaglia, Eleonora Aronica, Alessandra Morano, Pierangelo Cifelli, and Eleonora Palma. GABAergic Neurotransmission in Human Tissues Is Modulated by Cannabidiol. Life (2022)
  • Gabriele Ruffolo, Veronica Alfano, Alessia Romagnolo, Till Zimmer, James D. Mills, Pierangelo Cifelli, Alessandro Gaeta, Alessandra Morano, Jasper Anink, Angelika Mühlebner, Annamaria Vezzani, Eleonora Aronica & Eleonora Palma. GABAA receptor function is enhanced by Interleukin-10 in human epileptogenic gangliogliomas and its effect is counteracted by Interleukin-1β. Scientific Reports (2022)
  • Alessandra Morano, Emanuele Cerulli Irelli, Enrico M. Salamone, Biagio Orlando, Martina Fanella, Emanuele Tinelli, Gabriele Ruffolo, Luigi Zuliani, JinaneFattouch, Mario Manfredi, Anna T. Giallonardo, Carlo Di Bonaventura. Late-onset seizures and epilepsy: electro-clinical features suggestive of autoimmune etiology. Frontiers in Neurology. (2022)
  • Veronica Alfano, Alessia Romagnolo, James D. Mills, Pierangelo Cifelli, Alessandro Gaeta, Alessandra Morano, Angelika Mühlebner, Eleonora Aronica, Eleonora Palma & Gabriele Ruffolo*. Unexpected effect of IL-1beta on the function of GABAA receptors in pediatric focal cortical dysplasia. Brain Sciences (2022)(*last author)
  • Stefania Scalise, Clara Zannino, Valeria Lucchino, Michela Lo Conte, Luana Scaramuzzino, Pierangelo Cifelli, Tiziano D’Andrea, Katiuscia Martinello, Sergio Fucile, Eleonora Palma, Antonio Gambardella, Gabriele Ruffolo*, Giovanni Cuda, and Elvira Immacolata Parrotta. Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity. Biomedicines (2022) (*corresponding author)
 
  • ApostolosMikroulis, Marco Ledri ,Gabriele Ruffolo, Eleonora Palma Gunther Sperk, Jesmond Dalli, Annamaria VezzaniA, MerabKokaia. Lipid mediator n-3 docosapentaenoic acid-derived protectin D1 enhances synaptic inhibition of hippocampal principal neurons by interaction with a G-protein-coupled receptor. Faseb Journal (2022)
  • Alessandra Morano, Emanuele Cerulli Irelli, Martina Fanella, Biagio Orlando, Enrico Michele Salamone, Emanuele Tinelli, Gabriele Ruffolo, Luigi Zuliani, JinaneFattouch, Mario Manfredi, Anna Teresa Giallonardo, Carlo Di Bonaventura. Olfactory impairment in autoimmune encephalitis: another piece of the puzzle. Journal of Neurology (2022)
  • Marco Ceccanti, Laura Libonati, Gabriele Ruffolo, Pierangelo Cifelli, Federica Moret, Vittorio Frasca, Eleonora Palma, Maurizio Inghilleri, Chiara Cambieri. Effects of 3,4-diaminopyridine on myasthenia gravis: preliminary results of an open-label study. Frontiers in Pharmacology (2022)
  • Pierangelo Cifelli, Gabriele Ruffolo, Marco Ceccanti, Chiara Cambieri, Laura Libonati, Eleonora Palma, and Maurizio Inghilleri. Classical and Unexpected Effects of Ultra-Micronized PEA in Neuromuscular Function. Biomolecules (2022);
  • Cassandra Margotta; Paola Fabbrizio; Marco Ceccanti; Chiara Cambieri; Gabriele Ruffolo; Jessica D’Agostino; MariaChiara Trolese; Pierangelo Cifelli; Veronica Alfano; Christian Laurini; Silvia Scaricamazza; Alberto Ferri; Gianni Sorarù; Eleonora Palma; Maurizio Inghilleri; Caterina Bendotti; Giovanni Nardo. Immune-mediated myogenesis and acetylcholine receptor clustering promote slow disease progression in ALS mouse models. Inflammation and neurodegeneration (2023)
  • Miriam Sciaccaluga*, Gabriele Ruffolo*, Eleonora Palma, Cinzia Costa. Conventional and unconventional anti-seizures drugs targeting novel pathophysiological mechanisms: focus on neurodevelopment and neurodegeneration. (2023), Currentneuropharmacology
  • Cassandra Margotta, Paola Fabbrizio, Marco Ceccanti, Chiara Cambieri, Gabriele Ruffolo, Jessica D’Agostino, Maria Chiara Trolese, Pierangelo Cifelli, Veronica Alfano, Christian Laurini, Silvia Scaricamazza, Alberto Ferri, Gianni Sorarù, Eleonora Palma, Maurizio Inghilleri, Caterina Bendotti, Giovanni Nardo. Immune-mediated myogenesis and acetylcholine receptor clustering promote slow disease progression in ALS mouse models. Inflammation and Neurodegeneration (2023)
  • Antonella Tramutola, Simona Lanzillotta, Giuseppe Aceto, Sara Pagnotta, Gabriele Ruffolo, Pierangelo Cifelli, Federico Marini, Cristian Ripoli, Eleonora Palma, Claudio Grassi, Fabio Di Domenico, Marzia Perluigi, Eugenio Barone. Intranasal administration of KYCCSRK peptide rescues brain insulin signaling activation and reduces AD-like neuropathology in a mouse model for Down syndrome. Antioxidants (2023)

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